WASHINGTON (AP) — The United States on Tuesday sanctioned a son of Mexican drug lord Joaquin “El Chapo” Guzman, three members of the powerful Sinaloa cartel and two Mexican-based firms, alleging they trafficked fentanyl and other drugs into the U.S.

The sanctions came the day Mexican President Andrés Manuel López Obrador was to talk with President Joe Biden by phone about immigration and the fentanyl crisis.

The Treasury Department designated El Chapo's son Joaquin Guzman Lopez and others for financial sanctions, including a freeze on American-owned assets and bank accounts and a ban on Americans doing business with them. A Culiacan, Mexico, chemical and lab equipment company and a real estate business also were targeted for sanctions.

The latest penalties by the Treasury's Office of Foreign Assets Control follow a set of April fentanyl trafficking charges brought against three other Guzman sons, Ovidio Guzmán López, Jesús Alfredo Guzmán Salazar and Iván Archivaldo Guzmán Sálazar, known as the Chapitos, or Little Chapos, and two dozen members of the Sinaloa cartel.

Fentanyl is the deadliest drug in the U.S. today. The Centers for Disease Control and Prevention estimates that 71,000 people died in the U.S. from overdosing on synthetic opioids such as fentanyl in 2021, up from about 58,000 the year before.

López Obrador has denied that drug cartels make fentanyl in Mexico, although he has acknowledged that precursor chemicals and finished fentanyl are smuggled into Mexico from China, a claim China has denied.

Mexico and China are the primary sources for fentanyl and fentanyl-related substances trafficked directly into the U.S., according to the Drug Enforcement Administration, which is tasked with combating illicit drug trafficking.

Growing tensions between the U.S. and China on multiple fronts have made efforts to stop the importation of fentanyl more difficult, according to a Congressional Research Service report.

Most of the fentanyl trafficked in the United States comes from the Sinaloa cartel, the DEA says.

The Treasury’s undersecretary for terrorism and financial intelligence, Brian Nelson, said that the department “in close coordination with the Government of Mexico and U.S. law enforcement, will continue to leverage our authorities to isolate and disrupt Los Chapitos and the Sinaloa Cartel’s operations at every juncture.”

Guzman Lopez was not in custody Tuesday, and it was unclear if he had a lawyer who could speak on his behalf about the sanctions. A lawyer for El Chapo didn’t immediately return a message seeking comment.